Many clinically important drugs are natural products produced by bacteria and fungi. In particular, polyketide antibiotics have been found to be an extremely rich source of biologically active compounds with a broad range of pharmaceutical activities. Aromatic nitro groups are relatively rare functional groups in natural products including polyketide antibiotics but are found in diverse types of important antibiotics, such as chloramphenicol, pyrrolnitrin, aureothin, azomycin, and rufomycin. Surprisingly, the biosynthesis of aromatic nitro groups is poorly understood. To date, only two enzymes..